SIK1 Knockout cell line (EA.hy926)

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location： Home > Products > KO Cell Lines > SIK1 Knockout cell line (EA.hy926)

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SIK1 Knockout cell line (EA.hy926) Order

SIK1 Knockout cell line (EA.hy926)
Catalog#
YKO-H1153
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SIK1 Knockout cell line (EA.hy926)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SIK1 Knockout cell line（EA.hy926）
Cell Morphology
Endothelial cells, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
SIK1
Gene ID
150094
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 5 (based on transcript SIK1-201)
Validation: PCR+Sanger Sequencing
SIK1 Knockout cell line (EA.hy926) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:EA.hy926		 STR Info (Cell bank)
Cell Line:EA.hy 926
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 8910.08910.0
D16S539 11121112
VWA 14171417
TH01 689.3689.3
AMEL XXXX
TPOX 8989
CSF1PO 101112.0101112.0
D12S391 1518
FGA 2223
D2S1338 2224
D21S11 282932.0
D18S51 131517.0
D8S1179 1313
D3S1358 1516
D6S1043 111222.0
PENTAE 71112.0
D19S433 1314
PENTAD 911
D1S1656 1217
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the EA.hy926 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIK1 gene knockout SIK1 Knockout cell line（EA.hy926） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SIK1 Knockout cell line (EA.hy926)
SIK1 Knockout cell line (EA.hy926)
Catalog#
YKO-H1153
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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